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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
16/11/2018 |
Data da última atualização: |
06/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CONCEIÇÃO, A. A.; SOARES NETO, C. B.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; MILLER, R. N. G.; MENDONCA, S. |
Afiliação: |
APARECIDO ALMEIDA CONCEIÇÃO, Universidade Federal da Bahia; CLEMENTE BATISTA SOARES NETO, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; ROBERT NEIL GERARD MILLER, Universidade de Brasília; SIMONE MENDONCA, CNPAE. |
Título: |
Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Plos One, v. 13, n. 5, e0196164, May 2018. |
DOI: |
https://doi.org/10.1371/journal.pone.0196164 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus. MenosAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free ... Mostrar Tudo |
Palavras-Chave: |
Human-serum albumin; Performance liquid-chromatography; Terpenoid aldehydes. |
Thesaurus Nal: |
Biomass; Cottonseed cake; Gossypol; White-rot fungi. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222356/1/pone.0196164.pdf
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Marc: |
LEADER 02441naa a2200277 a 4500 001 2099518 005 2021-04-06 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1371/journal.pone.0196164$2DOI 100 1 $aCONCEIÇÃO, A. A. 245 $aDevelopment of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.$h[electronic resource] 260 $c2018 520 $aAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus. 650 $aBiomass 650 $aCottonseed cake 650 $aGossypol 650 $aWhite-rot fungi 653 $aHuman-serum albumin 653 $aPerformance liquid-chromatography 653 $aTerpenoid aldehydes 700 1 $aSOARES NETO, C. B. 700 1 $aRIBEIRO, J. A. de A. 700 1 $aSIQUEIRA, F. G. de 700 1 $aMILLER, R. N. G. 700 1 $aMENDONCA, S. 773 $tPlos One$gv. 13, n. 5, e0196164, May 2018.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registros recuperados : 20 | |
3. | | SILVA, L. da; MARCHIORI, P. E. R.; MACIEL, C. P.; MACHADO, E. C.; RIBEIRO, R. V. Fotossíntese, relações hídricas e crescimento de cafeeiros jovens em relação à disponibilidade de fósforo. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 9, p. 965-972, set. 2010 Título em inglês: Photosynthesis, water relations and growth of young coffee plants according to phosphorus availability.Biblioteca(s): Embrapa Unidades Centrais. |
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6. | | RIBEIRO, R. V.; SILVA, L. da; RAMOS, R. A.; ANDRADE, C. A. de; ZAMBROSI, F. C. B.; PEREIRA, S. P. O alto teor de silício no solo inibe o crescimento radicular de cafeeiros sem afetar as trocas gasosas foliares. Revista Brasileira de Ciência do Solo, Viçosa, v. 35, n. 3, p. 939-948, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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7. | | SILVA, I. D. S.; QUEIROGA, J. L. de; SILVA, L. da; GONÇALVES, L. P. C.; ALVAREZ, I. A. Experiências práticas de manejo orgânico de fruteiras no Sítio Catavento, Indaiatuba - SP. Cadernos de Agroecologia, v. 15, n. 2, 2020. Edição dos Anais do XI Congresso Brasileiro de Agroecologia, São Cristóvão, Sergipe, 2020. 5 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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9. | | ROSA, J. R. B. F.; GUIMARÃES, C. T.; MAGALHAES, J. V. de; DIAS, K. O. das G.; SILVA, L. da C. e; PASTINA, M. M. Aplicação da associação genômica no melhoramento de plantas. In: PEIXOTO, L. de A.; BHERING, L. L.; CRUZ, C. D. (ed.). Seleção genômica aplicada ao melhoramento genético. Viçosa, MG: Universidade Federal de Viçosa, 2022. p. 47-71.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Milho e Sorgo. |
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10. | | SILVA, I. D. S. S.; QUEIROGA, J. L. de; FAGUNDES, G. G.; MORICHITA, L. S. Y.; RAFAELA DE OLIVEIRA MINE; SILVA, L. DA; ALVAREZ, I. A. Análise da viabilidade da fruticultura orgânica no Circuito das Frutas, Estado de São Paulo. Revista dos Trabalhos de Iniciação Científica da UNICAMP, Campinas, SP, n.26 ,out. 2018. 1 p.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Territorial. |
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11. | | MELO, I. S. de; SOUZA, W. R.; SILVA, L. da; SANTOS, S. N.; ASSALIN, M. R.; ZUCCHI, T. D.; QUEIROZ, S. C. do N. de. Isolation and characterization of a novel Pseudomonas sp. strain from Deschampsia antarctica as a potential biocontrol agent. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 15., 2014, Seoul. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2014. p. 139.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
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12. | | GUIMARÃES, C. T.; SILVA, L. da C.; MENDES, F. F.; PASTINA, M. M.; SOUZA, I. R. P. de; DAMASCENO, C. M. B. Mapeamento de QTLs e seleção assistida por marcadores moleculares. In: DELIMA, R. O.; BORÉM, A. (Ed.). Melhoramento de milho. Viçosa, MG: Universidade Federal de Viçosa, 2018. cap. 13, p. 307-328.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Milho e Sorgo. |
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13. | | SILVEIRA, M. H. L. S.; SIQUEIRA, F. G. de; RAU, M.; SILVA, L. da; MOREIRA, L. R. de S.; FILHO, E. X. F.; ANDREAUS, J. Hydrolysis of sugarcane bagasse with enzyme preparations from Acrophialophora nainiana grown on different carbon sources. Biocatalysis and Biotransformation, v. 32, n. 1, p. 53-63, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Agroenergia. |
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14. | | AZEVEDO, G. C.; CHEAVEGATTI-GIANOTTO, A.; NEGRI, B. N.; SILVA, L. da C. e; MAGALHAES, J. V.; GARCIA, A. A. F.; LANA, U. G. P.; SOUSA, S. M. de; GUIMARAES, C. T. QTL mapping and identification of putative PSTOL homologs associated with phosphorus acquisition traits in maize seedlings. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 232.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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15. | | AZEVEDO, G. C.; CHEAVEGATTI-GANOTTO, A.; NEGRI, B. F.; HUFNAGEL, B.; SILVA, L. da C. e; MAGALHAES, J. V.; GARCIA, A. A. F.; LANA, U. G. P.; SOUSA, S. M. de; GUIMARAES, C. T. Multiple interval QTL mapping and searching for PSTOL1 homologs associated with root morphology, biomass accumulation and phosphorus content in maize seedlings under low-P. BMC Plant Biology, v. 15, n. 172, p. 1-17, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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16. | | PASTINA, M. M.; SILVA, R. R.; GUIMARAES, L. J. M.; GUIMARAES, C. T.; DIAS, K. O. das G.; SILVA, L. da C. e; MAGALHAES, J. V. de; GUIMARAES, P. E. de O.; PARENTONI, S. N.; GARCIA, A. A. F. Modelos GBLUP univariados e multivariados para seleção genômica para tolerância ao déficit hídrico em milho. Sete Lagoas: Embrapa Milho e Sorgo, 2016. 12 p. (Embrapa Milho e Sorgo. Circular Técnica, 223).Biblioteca(s): Embrapa Milho e Sorgo. |
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17. | | SOUZA, V. F. de; PEREIRA, G. da S.; PASTINA, M. M.; PARRELLA, R. A. da C.; SIMEONE, M. L. F.; BARROS, B. de A.; NODA, R. W.; SILVA, L. da C. e; MAGALHAES, J. V. de; SCHAFFERT, R. E.; GARCIA, A. A. F.; DAMASCENO, C. M. B. QTL mapping for bioenergy traits in sweet sorghum recombinant inbred lines. G3: Genes, Genomes, Genetics, v. 11, 112021, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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18. | | BERNARDINO, K. C.; PASTINA, M. M.; MENEZES, C. B. de; SOUSA, S. M. de; MACIEL, L. S.; CARVALHO JÚNIOR, G.; GUIMARÃES, C. T.; BARROS, B. de A.; SILVA, L. da C. e; CARNEIRO, P. C. S.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. de. The genetic architecture of phosphorus efficiency in sorghum involves pleiotropic QTL for root morphology and grain yield under low phosphorus availability in the soil. BMC Plant Biology, v. 19, n. 87, p. 1-15, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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19. | | DIAS, K. O. das G.; GEZAN, S. A.; GUIMARÃES, C. T.; NAZARIAN, A.; SILVA, L. da C. e; PARENTONI, S. N.; GUIMARAES, P. E. de O.; ANONI, C. de O.; PÁDUA, J. M. V.; PINTO, M. de O.; NODA, R. W.; RIBEIRO, C. A. G.; MAGALHAES, J. V. de; GARCIA, A. A. F.; SOUZA, J. C. de; GUIMARAES, L. J. M.; PASTINA, M. M. Improving accuracies of genomic predictions for drought tolerance in maize by joint modeling of additive and dominance effects in multi-environment trials. Heredity, London, v. 121, n. 1, p. 24-37, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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20. | | NASCIMENTO, R. Q.; RIBEIRO, C. V. D. M.; COLAUTO, N. B.; SILVA, L. da; LEMOS, P. V. F.; FERREIRA, E. de S.; LINDE, G. A.; MACHADO, B. A. S.; TAVARES, P. P. L. G.; BIASOTO, A. C. T.; GUEZ, M. A. U.; CARVALHO, N.; ASSIS, D. de J.; SILVA, J. B. A. da; SOUZA, C. O. de. Utilization of agro-industrial residues in the rearing and nutritional enrichment of Zophobas atratus larvae: new food raw materials. Molecules, v. 27, n. 20, article 6963,2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 20 | |
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